LabTurbo
LabTurbo is a spin column open design for different kinds of nucleic acid purification; it offers complete solutions from barcode reader, primary tube load, sample homogenization, lyses, bind, wash, removal of residuals, elution, cooling storage, reaction (PCR) setup, video check, communication and documentation. It is a rapid and economic walkaway system; it takes about 75 minutes for 96 samples (LabTurbo 96). It allow users to use the original use manual kit just with addition of a column adaptor and a non-organic clean buffer. So users can use their original manual kits.
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Highest Recovery
10-fold dilutions of Dengue virus RNA (from 10-3 to 10-8) were purified by LabTurbo and analyzed by the Real Time PCR System |
Cross contamination free
In alternating positive and negative, there were no-cross contaminations for purifying 108 copies of serum of HBV by using nested PCR |
Effective removal of inhibitors
Analysis the presence of PCR inhibitors by HBV QPCR detection. LabTurbo's result shows significant to reduce of the Ct value with the increased template volume, but manual's did not. |
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High purity & reproducible yield
Genomic DNA were purified from 200 µl whole blood. Yields of DNA were determined by absorbance (A260). This results show the consistent of yields (ug) and purity(A260 / A280) (CV% < 5%) of 32 samples aliqouted from the same blood source. |
Consistency
QPCR detection of HBV DNA extracted from 36 repeats of HBV patient's serum sample (diluted 100 times in urine). The CV% of Ct value is 1.81%, shows high consistency. |
LabTurbo is a quality guarantee robotic workstation; it provides automation protocols with cross contamination-free and effective removal of inhibitors to avoid false positive and false negative results. The space of array between samples is allowed the robotic with multiple pipette go not to cross over other samples to avoid the cross contamination due to aerosol drops. There are a lot of inhibitors in the samples and in the reagents that have obviously negative effects on nucleic acid applications, especially for PCR reaction, LabTurbo offers an optimization wash protocol to effectively remove the inhibitors without reducing nucleic acid recovery.